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Biorank Laboratories
Biological Age Reversal Protocol
Educational purposes only. Not medical advice. Always consult your doctor before starting supplements.
Targeting the hallmarks of aging with senolytics, NAD+ restoration, autophagy activators, and mitophagy enhancers to reduce biological age
Alysa Analysis for
Biological Age Reversal Protocol
Priority Guide
Restores NAD+ levels that decline ~50% by middle age. NAD+ is essential for SIRT1/SIRT3-mediated DNA repair, epigenetic maintenance, and mitochondrial function. Human trials show improved vascular function and muscle performance in older adults.
Recommended Dose
500mg daily on empty stomach
Expected Timeframe
8-12 weeks for NAD+ restoration
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Flavonoid identified as the most potent natural senolytic in a Mayo Clinic screen. Selectively clears senescent (zombie) cells that secrete inflammatory SASP factors driving aging. Used in cycling protocol, not daily, to avoid tolerance.
Recommended Dose
500mg x 2 days on, then off for 1 month (senolytic cycling)
Expected Timeframe
Repeat monthly cycles; effects measured at 3-6 months
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Polyamine that is one of the strongest known dietary autophagy inducers. Autophagy declines with age, leading to accumulation of damaged proteins and organelles. Epidemiological data links higher spermidine intake to reduced cardiovascular mortality and extended lifespan.
Recommended Dose
1-5mg daily (or dietary: wheat germ, natto, aged cheese)
Expected Timeframe
Ongoing (long-term intervention)
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The most potent known natural mitophagy activator. Selectively removes damaged mitochondria so they can be replaced with functional ones. RCTs show improved mitochondrial function and muscle endurance in older adults. Branded form: Mitopure (Timeline Nutrition).
Recommended Dose
500-1000mg daily
Expected Timeframe
4-8 weeks for mitochondrial biomarkers
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Emerging Krebs cycle intermediate that declines with age. Calcium AKG extended lifespan 12% and compressed morbidity in mouse studies. May reduce epigenetic age. Human trial (Rejuvant) showed 8-year biological age reversal in preliminary data.
Recommended Dose
1000mg calcium alpha-ketoglutarate daily
Expected Timeframe
6-12 months for epigenetic clock changes
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
Always consult your doctor or qualified healthcare provider before starting any supplement regimen, especially if you have existing health conditions or take medications.
Supplements may interact with medications and are not intended to diagnose, treat, cure, or prevent any disease.
The evidence ratings reflect our analysis of published research and may not capture every nuance of individual health situations.